Method and product for decomposing organic compounds

ABSTRACT

A method for the biological decomposition of organic compounds of the group of hydrocarbons, fats, oils, waxes, and derivatives thereof as well as mixtures thereof in media polluted therewith, in the presence of at least one dispersant, wherein the medium polluted with the pollutants is treated with at least one dispersant and with microorganisms.

FIELD OF THE INVENTION

The present invention relates to the field of the biologicaldecomposition of organic compounds in media polluted therewith. Theinvention relates in particular to a method for the biologicaldecomposition of organic compounds of the group of hydrocarbons, fats,oils, waxes, and derivatives thereof as well as mixtures thereof inpolluted media, to a product to be used in this method, and to a numberof possible applications of the method and of the product.

BACKGROUND OF THE INVENTION

Very wide-spread classes of organic compounds are hydrocarbons whichinclude in particular petroleum. Petroleum consists of a mixture ofhydrocarbons, sulfur compounds, heterocyclic compounds, etc. Thesedifferent substances vary substantially with respect to theirproperties. The behavior of petroleum in the environment depends on thephysical properties thereof, such as boiling point and water solubility,the chemical properties, such as chemical and photochemicaldecomposability and toxicity, and the biological properties whichinclude biological decomposability and bio accumulation ability.

Petroleum that gets into the marine environment will first of all floaton the water surface since its specific weight is smaller than that ofsea water, and will form a more or less thick oil film there. By meansof wave movements and flows, a horizontal trans-port on the water takesplace in which the oil moreover spreads across the area. As a functionof the physical and chemical properties, parts of the oil film transitto the adjacent environment compartments such as air and water. Theextent and the rate of these transitions depend decisively on theprevailing environmental conditions such as, for instance, wind, waves,and temperature. The higher the temperature, the greater the windstrength and the higher the waves, the larger the share of oil thatevaporates or dissolves. Apart from these processes, however, some oilcomponents floating on the water surface may also be decomposedchemically and photochemically. The major portion of the oil is inaddition subject to a biological decomposition by autochthonous,specific oil-decomposing bacteria.

In other ecological regions, for instance, in soil, accidents occur timeand again during the exploitation, the transportation, the processing,and the storage of oil or oil products, wherein larger amounts of oil oroil products emanate from the pipelines, transporters, or containersintended for them and contaminate the surrounding soil. Here, too, theproblem arises that the oil contaminations modify their state under theinfluence of the atmospheric conditions in that easily volatilecomponents evaporate and the remaining, high-boiling and viscouscomponents increasingly clump together and resinify. In the course oftime, the oil in addition penetrates at various depths into the soilwith serious groundwater contamination being able to occur.

A number of methods for the conversion of petroleum products havealready been known. Thus, GB 1 354 543 describes, for instance, a methodfor converting a petroleum product to a biologically decomposableemulsion by mixing a specific microorganism nutrient solution and anorganic acid in water. Micro emulsions containing surfactants are knownfrom EP 1 113 863 B1. These micro emulsions act as accelerators for thebiological decomposition of hydrophobic pollutions, for instance,hydrocarbons. A composition on the basis of surfactants for thedispersion of hydrocarbons in water, in particular in sea water, isdescribed in GB 2 115 311 A. Finally, CA 2 229 761 C describes a methodfor the biological decomposition of a sludge on oil basis containing amixture of petroleum hydrocarbons. This method takes place in a reactor,wherein an aqueous solution is formed from an oil-in-water emulsion ofthe sludge on oil basis, a bacterial culture, and nutrients for thebacterial culture. This aqueous solution may contain a surfactant.

The use of a dispersant against oil pollutions in particular in marineareas results in a dissolution of the oil layer into very many littledroplets, so that the oil surface increases and decomposing processes byavailable bacteria are enhanced. Thereby, the autochthonous flora of therespective location is used. According to experience, this is, however,frequently not very lavish and, above all in acute cases, not adapted tothe location. Hence, there may arise substantial disturbances in theecological system. It is especially important to contain the effects ofoil catastrophes on the marine environment and also on such areas thatare strongly contaminated by oil contamination, for instance, soils.

Substantial damages to the environment also result from thecontamination with fats, oils, waxes, and derivatives thereof. In manyareas, fats, oils, waxes, and derivatives thereof which may disturb theecological balance are often discharged. Here, too, there existsconsiderable demand of removing such contaminations with ecologicallycompatible solutions.

It is therefore an object of the present invention to provide atechnology by means of which the decomposition of contaminants in mediapolluted with organic compounds is performed in an accelerated manner inan ecologically acceptable way, wherein such environments may be treatedwhich have only a weak and unadapted populating by autochthonousmicroorganisms.

It is also an object of the present invention to provide a product bywhich the user is capable of quickly and practically treat the pollutionby means of organic compounds directly on the spot.

SUMMARY OF THE INVENTION

The object of the invention is solved by the method with the features ofclaim 1 and by the combination product according to claim 19.Advantageous developments of the method and of the product result fromthe subclaims which further develop the idea of the present invention.

The present invention describes in a first aspect a method for thebiological decomposition of organic compounds of the group ofhydrocarbons, fats, oils, waxes, and derivatives thereof as well asmixtures thereof in media polluted therewith, in the presence of atleast one dispersant, characterized in that the medium polluted with theorganic compounds is treated with the at least one dispersant and withmicroorganisms.

The biological decomposition of the organic compounds in media pollutedtherewith is substantially accelerated by the method according to theinvention. The application with at least one dispersant results in adissolution of the organic compounds into many small droplets(micelles), so that decomposition processes, for instance, by bacteria,are promoted. The addition of the microorganisms results in a surprisingmanner in an increased microbial progeny rate of the microorganisms thatare capable of quickly influencing the pollutant and of accelerating itsdecomposition.

In accordance with a second aspect of the invention, a combinationproduct is provided which comprises a dispersant source and amicroorganisms source. This combination product enables quick acting inthe case of a suddenly occurring pollution with organic compounds inaqueous and non-aqueous media.

In a third aspect of the present invention, the method and thecombination product are used in aqueous and non-aqueous media pollutedwith organic compounds.

SHORT DESCRIPTION OF THE DRAWING

The only FIGURE shows a block diagram for explaining the microbiological growth in various cultures.

DETAILED DESCRIPTION OF THE PRESENT INVENTION

The method according to the invention is a method that renders itpossible to effect, even in media that are only weakly populated withautochthonous microorganisms, an accelerated decomposition of pollutantsbased on organic compounds. The method according to the invention mayeven be performed advantageously if the local microorganism flora isnormally not or just slowly self-decomposable.

The combinatory use of at least one dispersant and of the microorganismsis, depending on the kind and degree of contamination, performed in atwo-step mode. In a preferred embodiment of the method according to theinvention, the dispersant is first put in the medium polluted with theorganic compounds, and subsequently the microorganisms are added. Inanother preferred alternative of the method according to the invention,the microorganisms are first put in the medium polluted with the organiccompounds, and subsequently the dispersant is added.

It is further also possible to use microorganisms and dispersantsimultaneously.

The hydrocarbons to be decomposed by means of the method according tothe invention are preferably oils and oil products such as, forinstance, petroleum distillates, mainly the heavy distillates containingheavy polyaromatic compounds, their derivatives and further processedproducts. These petroleum products and derivatives are, as a rule, notself-decomposable if they get into the environment. Thus, the mostimportant criterion is the biological decomposability of these petroleumproducts. It determines the residence time in the environment. Thequicker decomposition takes place, the less is the probability ofaccumulation or the effect of possible toxic properties.

By means of the method according to the invention, such organiccompounds that rank among the fats, oils (fat oils), waxes, andderivatives thereof are also decomposed biologically. These are fats,oils, and waxes of herbal and animal origin. Synthetic fats, oils, andwaxes are also included. Furthermore, mixtures of fats, oils, and waxesare also comprised.

In a preferred embodiment of the method according to the invention, atleast one surfactant is used as a dispersant, said surfactant beingpreferably selected from ionic, non-ionic, and amphoteric surfactants,but often mixtures thereof. The dispersant present an HLB value, whichis a Hydrophylic Lipophylic Balance, of about 8 to 10.

The ionic surfactants comprise anionic and cationic surfactants. Anionicsurfactants are, for instance, soaps, carboxylates, sulfonates, and,sulfates. The cationic surfactants comprise, for instance, amine saltsand quaternary ammonium compounds.

Examples of non-ionic surfactants that may be used are ethoxylates,sucrose esters, aminoxides. Amphoteric surfactants that may be used are,for instance, betaines and sultaines.

In a particular embodiment of the method according to the invention, thedispersant comprises:

-   -   about 40 to 65 wt % surfactant combination comprising        -   a) a blend of sorbitan monooleate and sorbitan monooleate            polyoxyethylene adduct, having an HLB of about 8.75 to 9.5,        -   b) eventually a blend of sorbitan trioleate and sorbitan            monooleate polyoxyethylene adduct having an HLB of about 8            to 10, wherein the weight ratio of the blend (a) to the            blend (b) is within the range of about 0.5:1 and 6:1,    -   about 35 to 60 wt % wetting agent comprising at least one        surface active water dispersable dialkysulfosuccinate, said        percentage being based on dry salt.

In preferred embodiment, the dispersant is used in admixture withpetroleum distillates. More preferably, the dispersant is used inadmixture with water and light distillate, the final compositioncontaining from 25 to 60% dispersant, 1 to 20% water and 5 to 74% lightdistillate.

Light distillate means dearomatized kerosin, in all or in part withpolyalcoholic ethers, preferably polyalkylglycol ethers. Most relevantdispersants are disclosed by reference to patents EP254704 and EP398860. Preferred dispersants are FINASOL OSR51 and FINASOL OSR52available commercially available by Total Fluides.

Generally, the dispersant is added to the medium polluted with theorganic compounds in such an amount that it can sufficiently bedispersed. It has turned out in practice that the dispersant is used ina concentration of 1 to 10%, relating to the amount of the organiccompounds in the polluted medium. In a particularly preferredembodiment, the dispersant is used in a concentration of 3 to 7%,relating to the amount of the organic compounds in the polluted medium.

In accordance with the method of the invention, the microorganisms areadded to the polluted medium in the form of an aqueous solution. Thesemicroorganisms contribute substantially to the fact that thedecomposition rate of the pollutant is accelerated.

As microorganisms, bacteria, yeasts, fungi, and mixtures thereof may beused in the method according to the invention. Furthermore, mixtures ofbacteria, yeasts, and fungi among each other are also possible.

In the method according to the invention, cells of the generaacinetobacter, alicaligenes, bacillus, pseudomonas, alcanivorax,cycloclasticus, marinobacter, neptunomonas, oleiphilus, oleispira,and/or thalassolitus are preferably used as bacteria. Preferred cellsare those of the stems Pseudomonas marginalis, P. grimontii, P. veroniiand P. azotoformans in aqueous solution.

Examples of fungi to be used in accordance with the invention are cellsof the genera phanerochaete, aspergillus, penicillium, fusarium,amorphoteca, neosartorya, paleomyces, talaromyces, and/or graphium,prepared in aqueous solution (“submerse culture”). A preferred genus isPenicillium.

Preferred yeasts are cells of the genus candida.

In a preferred embodiment of the method according to the invention, themicroorganisms are used in a concentration of 10³ to 10¹⁵ CFU/ml,related to the amount of organic compounds in the polluted medium. In aparticularly preferred embodiment, the microorganisms are used in aconcentration of 10⁷ to 10¹² CFU/ml, related to the amount ofhydrocarbons in the polluted medium.

In practice, there are various possibilities of application of thedispersant and the microorganisms on the polluted medium. In a preferredembodiment of the present invention, the dispersant and themicroorganisms are sprayed on alone one after the other or together. Inthe case of larger contaminated areas it is possible to apply eitherefficiently by airplanes or from boats. In the case of smallercontaminated areas, the use of hand-held sprayers is sufficient.

Another possibility consists in applying the microorganisms and thedispersant with a dosing pump onto the contaminated areas.

Depending on the kind and degree of medium contamination with theorganic compounds, the addition of the microorganisms and/or thedispersant may be performed directly or time-delayed one after the otheron the polluted medium. In some case the microorganisms are sprayedfirst, in other case, the dispersant is sprayed first.

The polluted media adapted to be treated with the method according tothe invention are aqueous and non-aqueous areas, surfaces, andpunctually polluted locations.

The aqueous media comprise, for instance, polluted open seas, inlandwaters, cisterns, and waste water treatment plants.

Non-aqueous media suited to be treated with the method according to theinvention relate to polluted areas and surfaces such as, for instance,soils in contaminated industrial sites, gas stations, and gastronomysites.

In the present case of the treatment of non-aqueous media, care has tobe taken that a corresponding amount of aqueous medium, e.g. water, isavailable to obtain a field capacity of approximately 50% in non aqueousmedia. The man skilled in the art is able to determine the necessaryamount of aqueous medium needed and to use it correspondingly to providethe optimal media moisture. It will also depend on the degree of thecontamination and on the nature of the organic compounds that causedthis contamination.

The present invention also describes a combination product comprising adispersant source and a microorganisms source as components.

The dispersant source of the combination product is a surfactantselected from ionic, non-ionic, and amphoteric surfactants as well asmixtures thereof. Examples of ionic, non-ionic, and amphotericsurfactants have already been described above.

In a preferred embodiment of the combination product according to theinvention, the dispersant source contains one or more dispersant (s)which is/are available as a mixture with light distillate and possiblywater. Preferred mixtures or examples, respectively, have already beenmentioned initially.

In the combination product, the microorganisms source containsmicroorganisms which are preferably available as a solution, forinstance, in an aqueous solution. The microorganisms are selected as afunction of the kind of contamination with the organic compounds, thedegree of contamination, and the size of the contaminated area. They areselected from bacteria, yeasts, fungi, and mixtures thereof. Bacteriamixtures are preferred. Examples of preferred bacteria, yeasts, fungifor the bacteria solution or the bacteria material, as microorganismssource, have already been disclosed above.

The method according to the invention and the combination productaccording to the invention may be used successfully in aqueous andnon-aqueous media for the efficient decomposition of organic compoundsin contaminated areas, these organic compounds being of the group ofhydrocarbons, fats, oils, waxes, and derivatives thereof as well asmixtures thereof. It was found that the dispersant according to theinvention favours the availability for the microorganisms either in themicelle or about the micelle. After all, the decomposition rate of thepollutant is substantially increased by the addition of themicroorganisms.

The method and the combination product according to the invention isvery successful for open seas, inland waters, cisterns, and waste watertreatment plants which are deeply polluted or contaminated by organiccompounds, specifically heavy polyaromatic compounds which remain afterevaporation of lighter hydrocarbon compounds. The method and thecombination product according to the invention may especially be used inan extremely efficient manner in the case of oil tanker damages or oilplatform damages so as to fight the effects of oil accidents and topreserve the ecological system.

The method and the combination product according to the invention mayalso be used in non-aqueous media polluted with organic compounds.Examples of non-aqueous media are polluted areas and surfaces, such assoils in contaminated industrial sites, e.g. junk yards, old chemical orpetroleum industrial sites, tank farms, gas stations, and gastronomysites.

The present invention will now be explained in detail by means of theexamples, wherein the examples are in no way suited to restrict thepresent invention only to the embodiments described there.

EXAMPLES Example 1

For the illustration of the microbial growth on a defined carbon source,a minimal medium without carbon source was generated:

Na₂HPO₄ 7.2 g KH₂PO₄ 3.0 g NaCl 0.5 g NH₄Cl 1.0 g Dist. water 1000 ml

The solution was set to a value of pH 7.4 with NaOH or HCl,respectively. After the autoclaving,

1M MgSO₄ 2.0 ml 2M CaCl₂ 0.1 mlwere added.

The medium was distributed to six cultures with 200 ml in suitablecontainers.

As microorganisms, two different microorganism mixtures MO1 (aqueoussolution containing Pseudomonas azotoformans, P. grimontii and P.marginalis) and MO2 (aqueous solution containing Penicillium sp. andPseudomonas azotoformans, P. grimontii and P. marginalis) by the companyInocre® were used. The consortia (Mo1 and MO2) of the microorganismswere produced as follows: The stems described above were grown inmineral medium (minimal medium see above with 1% (wt) fructose as carbonsource) as pure cultures up to cell numbers of more than 10⁸ CFU/ml Thepure cultures were mixed together in equal quantities.

The microorganism mixtures were set to cell numbers in the range of 10⁸to 10⁹ CFU/ml in aqueous solution.

Diesel oil in a concentration of 1% (v/v) in the minimal medium was usedas a carbon source and simulation for the hydrocarbon contamination.

As a dispersant available in admixture with light distillate, FinasolOSR 52 (Total Fluides) was added to the minimal medium in aconcentration of 5% (w/v) (50 g Finasol OSR 52 per 1000 ml minimalmedium).

Example 2

Cultures with 200 ml minimal medium each were produced. Depending on theproblem, the following components were added:

-   a) To determine the effect of Finasol only on a diesel oil layer, a    culture was polluted with 2 ml diesel oil and subsequently sprayed    with 0.1 g (5% (w/v)) Finasol.-   b) To test the effect of the microorganism mixture 1 (MO1) only on    diesel oil, a culture was polluted with 2 ml diesel oil and    subsequently sprayed with 0.1 ml of MO1 with 10⁸ CFU/ml in aqueous    solution.-   c) To test the joint effect of Finasol and MO1 on diesel oil, a    culture was polluted with 2 ml diesel oil, sprayed with 0.1 ml MO1    (10⁸ CFU/ml in aqueous solution), and subsequently superimposed with    0.1 g Finasol.-   d) To test the joint effect of Finasol and a second microorganism    mixture (MO2) on diesel oil, a culture was polluted with 2 ml diesel    oil, sprayed with 0.1 ml MO2 (10⁹ CFU/ml), and subsequently    superimposed with 0.1 g Finasol.-   e) To determine whether Finasol is microbiologically decomposable,    0.1 g Finasol and 0.1 ml MO1 (10⁸ CFU/ml) were added to a culture    without diesel oil.-   f) To determine whether Finasol is microbiologically decomposable,    0.1 g Finasol and 0.1 ml MO2 (10⁹ CFU/ml) were added to a culture    without diesel oil.

The cultures were incubated under stirring for one week. The samplingsfor the determination of the microbiological growth took place on day 2and on day 7.

Example 3

The interpretation of the experiments took place pursuant to visualparameters and by the determination of the microbiological growth.Microbial growth is quantified as colony forming units (CFU/ml), seeFIG. 1

1. Visual Evaluation

-   -   The visible effects of the experiments were documented on day 2        and day 7. The following resulted for the various cultures:

-   a) Day 2: distinct clouding, corresponding to fine diesel oil    droplets distribution obtained by Diesel dispersion in presence of    Finasol.    -   Day 7 like day 2

-   b) Day 2: culture clear    -   Day 7: distinct clouding, caused by the growth of MO1,        sedimented biomass can be recognized.

-   c) Day 2: distinct clouding, corresponding to fine diesel oil    droplets distribution obtained by Diesel dispersion in presence of    Finasol.    -   Day 7 like day 2 d) Day 2: distinct clouding, corresponding to        fine diesel oil droplets distribution obtained by Diesel        dispersion in presence of Finasol.    -   Day 7 like day 2, but slight bio film can be recognized at the        rim of the vessel.

-   e) Day 2: distinct clouding, corresponding to fine diesel oil    droplets distribution obtained by Diesel dispersion in presence of    Finasol.    -   Day 7 like day 2

-   f) Day 2: distinct clouding, corresponding to fine diesel oil    droplets distribution obtained by Diesel dispersion in presence of    Finasol.    -   Day 7 like day 2

By the visual evaluation, the dispersing properties of Finasol becomevery clear. The surface increase that is generated this way has apositive effect on the microbiological growth since a betteravailability of the carbons is thus given.

2. Microbiological Growth

The microbiological growth was tracked by the number of the colonyforming units (CFU) on CASO Agar.

a) c) d) e) f) Diesel + Finasol b) Diesel + MO1 Diesel + Finasol + MO1Diesel + Finasol + MO2 Finasol + MO1 Finasol + MO2 Day 2 0 0 2.5 × 10⁷8.7 × 10⁸  3.9 × 10⁴   3 × 10⁴ Day 7 0 1.4 × 10¹¹   4 × 10⁹ 1.1 × 10¹¹2.4 × 10⁸ 5.8 × 10⁸

FIG. 1 illustrates the microbiological growth of the cultures a) to f)by means of the CFUs.

The cell number determination revealed that MO1 without Finasol isindeed absolutely capable of using the diesel oil as a sole carbonsource and thus decomposing it (culture b). However, with a time delayonly. If, however, the microorganisms are used in combination withFinasol, a strong growth is generated much more quickly. Thedecomposition rate is drastically increased by a joint use (cultures cand d). The biological decomposability of Finasol by the microorganismmixtures is also given. The microorganisms, however, require as desiredat least the duration of one week to achieve efficient cell numbers(cultures e and f). Thus, the microorganisms have sufficient time to usethe surface increase of Finasol to decompose the pollutant before theFinasol is also transformed.

Disclosed is a method for the biological decomposition of organiccompounds of the group of hydrocarbons, fats, oils, waxes, andderivatives thereof as well as mixtures thereof in media pollutedtherewith, in the presence of at least one dispersant, wherein themedium polluted with the pollutants is treated with the at least onedispersant and with microorganisms.

1-29. (canceled)
 30. A method for biological decomposition of organiccompounds of the group of hydrocarbons, fats, oils, waxes, andderivatives thereof as well as mixtures thereof in media pollutedtherewith, in the presence of at least one dispersant wherein the mediumpolluted with the organic compounds is polluted non-aqueous media and atleast one dispersant having an HLB value of about 8 to 10 used as amixture with petroleum destillates is put in the medium polluted withthe organic compound, and subsequently microorganisms are added.
 31. Themethod according to claim 30, wherein a surfactant selected from ionic,non-ionic and amphoteric surfactants as well as mixtures thereof is usedas a dispersant.
 32. The method according to claim 30, wherein thedispersant comprises: about 40 to 65 wt % surface active agentcombination comprising a) a blend of sorbitan monooleate and sorbitanmonooleate polyoxyethylene adduct, having an HLB of about 8.75 to 9.5,b) eventually a blend of sorbitan trioleate and sorbitan monooleatepolyoxyethylene adduct having an HLB of about 8 to 10, wherein theweight ratio of the blend (a) to the blend (b) is within the range ofabout 0, 5:1 and 6:1, about 35 to 60 wt % wetting agent comprising atleast one surface active water dispersable dialkysulfosuccinate, saidpercentage being based on dry salt.
 33. The method according to claim 30wherein the dispersant is used in admixture with water and lightdistillate, the final composition containing from 25 to 60% dispersant,1 to 20% water and 5 to 74% light distillate.
 34. The method accordingto claim 33, wherein the light distillate is dearomitized kerosin, takenalone or in admixture with polyalcoholic ethers, preferablypolyalkylglycol ethers.
 35. The method according to claim 30, whereinthe dispersant is used in a concentration of 1 to 10 wt %, related tothe amount of the organic compounds in the polluted medium.
 36. Themethod according to claim 35, wherein the dispersant is used in aconcentration of 3 to 7 wt %, related to the amount of the organiccompounds in the polluted medium.
 37. The method according to claim 30,wherein an aqueous solution of microorganisms is used.
 38. The methodaccording to claim 37, wherein bacteria, yeasts, fungi, and mixturesthereof are used as microorganisms.
 39. The method according to claim38, wherein cells of the genera acinetobacter, alicaligenes, bacillus,pseudomonas, alcanivorax, cycloclasticus, marinobacter, neptunomonas,oleiphilus, oleispira, and/or thalassolitus are used as bacteria. 40.The method according to claim 39, wherein cells of the generaphanerochaete, aspergillus, penicillium, fusarium, amorphoteca,neosartorya, paleomyces, talaromyces, and/or graphium are used as fungi.41. The method according to claim 38, wherein cells of the genus candidaare used as yeast.
 42. The method according to claim 38, wherein themicroorganisms are used in a concentration of 10³ to 10¹⁵ CFU/ml ofpolluted medium, related to the amount of the organic compounds in thepolluted medium.
 43. The method according to claim 42, wherein themicroorganisms are used in a concentration of 10⁷ to 10¹² CFU/ml ofpolluted medium, related to the amount of the organic compounds in thepolluted medium.
 44. The method according to claim 30, wherein thedispersant and the microorganisms are sprayed on the polluted medium.45. A combination product that comprises the following components: adispersant source available as a mixture with light petroleum distillateand possibly water, and a microorganisms source.
 46. The productaccording to claim 45, wherein the dispersant is a surfactant selectedfrom ionic, non-ionic, and amphoteric surfactants as well as mixturesthereof.
 47. The product according to claim 45, wherein themicroorganisms are available as an aqueous solution.
 48. The productaccording to claim 47, wherein the microorganisms are bacteria, yeasts,fungi, and mixtures thereof.
 49. The product according to claim 48,wherein the bacteria are cells of the genera acinetobacter,alicaligenes, bacillus, pseudomonas, alcanivorax, cycloclasticus,marinobacter, neptunomonas, oleiphilus, oleispira, and/or thalassolitus.50. The product according to claim 48, wherein the fungi are cells ofthe genera phanerochaete, aspergillus, penicillium, fusarium,amorphoteca, neosartorya, paleomyces, talaromyces, and/or graphium. 51.The product according to claim 50, wherein the yeasts are cells of thegenus candida.
 52. The application of a method according to any of claim30 and of a combination product, in non-aqueous media polluted withorganic compounds, that comprises the following components: a dispersantsource available as a mixture with light petroleum distillate andpossibly water, and a microorganisms source.
 53. An applicationaccording to claim 52, wherein the non-aqueous media are polluted areasand surfaces in contaminated industrial sites, gas stations, andgastronomy sites.